High throughput sequencing technologies are revolutionizing genetic research. With this ‘‘rise of the machines’’, genomic sequences can be obtained even for unknown genomes within a short time and for reasonable costs. This has enabled evolutionary biologists studying genetically unexplored species to identify molecular markers or genomic regions of interest (e.g. micro- and minisatellites, mitochondrial and nuclear genes) by sequencing only a fraction of the genome. However, when using such datasets from non-model species, it is possible that DNA from non-target contaminant species such as bacteria, viruses, fungi, or other eukaryotic organisms may complicate the interpretation of the results. In this study weanalysed 14 genomic pyrosequencing libraries of aquatic non-model taxa from four major evolutionary lineages. Wequantified the amount of suitable micro- and minisatellites, mitochondrial genomes, known nuclear genes and transposable elements and searched for contamination from various sources using bioinformatic approaches. Our results show that in all sequence libraries with estimated coverage of about 0.02–25%, many appropriate micro- and minisatellites, mitochondrial gene sequences and nuclear genes from different KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways could be identified and characterized. These can serve as markers for phylogenetic and population genetic analyses. A central finding of our study is that several genomic libraries suffered from different biases owing to non-target DNA or mobile elements. Inparticular, viruses, bacteria or eukaryote endosymbionts contributed significantly (up to 10%) to some of the libraries analysed. If not identified as such, genetic markers developed from high-throughput sequencing data for non-model organisms may bias evolutionary studies or fail completely in experimental tests. In conclusion, our study demonstrates the enormous potential of low-coverage genome survey sequences and suggests bioinformatic analysis workflows. The results also advise a more sophisticated filtering for problematic sequences and non-target genome sequences prior to developing markers.
The resounding Conservative victory over Labour and the Liberal Democrats in the General Election has been warmly welcomed by the property industry this morning, with many predicting a post-election ‘super bounce’.Economic and political uncertainty has stalked the housing market since the EU Referendum and both big data and agents on the ground have highlighted the need for greater certainty of the UK’s economic direction to persuade people to start moving home again.Prior to the election, RICS described the property market as ‘subdued’ and ‘haunted’ by a lack of clarity over Brexit.Here’s the reaction to the Conservative’s landslide victory early this morning, which we will be adding to as the comments roll in.Bounce in confidenceIain McKenzie, CEO of The Guild of Property Professionals “Now that there has been a decisive win for the Conservatives and therefore a clear path forward has been established, I believe we will see a bounce in confidence and activity in the property market.“Many buyers and sellers have put their lives on hold waiting for an outcome to the political controversy playing out in front of us. This has led to a severe lack of stock coming onto the market as sellers have held back. The result of the general election has finally provided the population with clarity regarding the Brexit hurdle and we will almost certainly leave the EU at the end of January, meaning we can move forward as a country and the market can settle into a new normal, which should see activity start to improve as buyers and sellers return to the market.”Reassure the property marketNick Leeming, Chairman at Jackson-Stops“Over the last few years, both buyers and sellers have done well to adjust to the ongoing uncertainty facing our country, yet we hope that today’s result will finally provide some reassurance to the property market.“Throughout the Conservative party’s campaign, they pledged their support for greater home ownership and so now is the time for them to form a Government that will deliver on this.“In the lead up to Boris being elected Prime Minister, he spoke widely about stamp duty cuts for UK residents, yet this quickly fell by the wayside as he settled in to No.10.“Our latest research found that 41% of consumers think there should be a wholesale reduction in stamp duty across all price brackets, while more than a quarter think Government should abolish stamp duty on all homes under £500,000. Just 3% felt no change was required, which highlights the need for change.”Turbocharge the marketDavid Westgate, Group Chief Executive at agency chain Andrews Property Group“The property market will be one of the main beneficiaries of Friday’s decisive General Election result.“Such a conclusive victory for Boris Johnson has the potential to turbocharge the property market and get it out of its current rut.“The certainty that comes with a five-year administration will create confidence and bring back the aspirational buyers and sellers that have been lacking since the EU Referendum result.“For three years the property market has been gripped by political uncertainty and deadlock but now it can finally move on. There’s every chance we are now at the beginning of a market cycle that may not peak until 2027 or beyond, with growth of around 4% a year.“Let’s not kid ourselves that the housing crisis will be miraculously solved overnight, because it won’t. We now need a Housing Minister with a properly costed and fully structured long-term plan that looks beyond building a certain number of homes each year.“The structural issues in the property market will still be there when the euphoria of the Conservatives has faded.”Immediate bounceSebastian Kalmar, Residential Director at estate agency KALMARs “The result of the general election and the strong Conservative position should benefit the property market, as we now look towards greater clarity regarding the UK’s departure from the European Union and less uncertainty for buyers in the market.“For many buyers, the question of Brexit has put their purchasing plans on hold, but we expect a renewed surge in people committing to homes across London in the New Year.”Pent up demandGuy Gittins, Managing Director, Chestertons“If there’s one thing that slows the property market more than anything, its uncertainty and this morning, two big uncertainties have been removed: we will have a Conservative government for the next five years and we will be leaving the EU, with or without a deal.“We expect that the considerable pent-up buyer demand which has been waiting for Brexit clarity will now be released. Sellers will in turn be encouraged by the increase in demand and are likely to start putting their properties on the market in greater numbers and the increase in sales could see prices bounce back quite quickly.“We have already seen how quickly confidence can rebound with the pound surging to its highest level since June last year and the FTSE 250 hitting record highs, and buyers should consider acting sooner rather than later while prices are still at attractive levels compared the last market peak.”Full in-tray of decisionsRichard Donnell, Research Director at Zoopla “While voters ranked housing as the seventh most important issue for them as they went to the polls, the incoming Government faces an in-tray full of housing-related policy decisions.“The election result changes little when it comes to the fundamentals of the housing market. The challenges for housing vary across the country and there are no simple, national solutions.”Wait and seeFranz Doerr, CEO of alternative deposits platform flatfair“We ‘ll have to wait to see whether the Tories will create lifetime deposits as suggested in their manifesto.“While in practice this is a flawed idea as it does not provide a solution for the widespread issues faced between tenancies, we welcome the efforts of tackling the very real problem of hefty upfront rental costs.“The need to gather a cash deposit is an enormous barrier to renting and this won’t be avoided simply through deposit passporting.“The technology to make renting fairer for everyone is already available with platforms like flatfair which already allow tenants to rent without a deposit, whilst offering greater protection for landlords from any damages or rent arrears.”Sense of certaintyMarc von Grundherr, Director of London lettings firm Benham and Reeves“Forget political alignments for the minute, as we finally have a sense of certainty on which we can move forward as a nation, and while the curtain is far from falling on the rollercoaster that has been our European departure, we should see a fair degree of positive property market stimulation as we enter into next year.“The sluggish one percent annual rates of house price growth that have plagued the market for the last few years should now give way to a far healthier three to four percent.”Unhindered route to deliveryMichael Stone, CEO of newbuild specialist Stone Real Estate“While the Government’s track record of late has been fairly admirable with some 241,000 new homes delivered this year, there’s always room for improvement, and now the election dust has settled we should hopefully see an unhindered route to delivery and a positive impact on social housing given that it is linked via S106 agreements.“We’ve seen many big housebuilders operate on a more hands-off basis of late, largely due to a lower rate of house price growth and a fear of financial underperformance in tough market conditions.“However, the new build sector has actually been the silver bullet against Brexit uncertainty with those opting to enter the fray rewarded with consistent levels of buyer demand and buoyant sold prices to match.“With things only about to get better, the new build sector can expect a busy time over the coming year as pent up market apprehension surrounding our political landscape is relieved to a degree, and more homes are built, more homes are bought, and market sentiment receives a well-needed boost. ”Depressed primeFraser Slater, CEO of property finding agency Ludgrove Property“Now that a Conservative majority has been confirmed two of the key factors that have depressed prime London property prices and transactions over the last few years; namely Britain’s withdrawal from the EU and political uncertainty have been removed.“The third negative factor – Stamp Duty – remains a millstone but one that is not without hope. Although an increase in Stamp Duty for overseas buyers was muted in the Conservative manifesto, we are somewhat sanguine that Stamp Duty may be reduced for domestic buyers in the February Budget. After all the PM, Deputy PM and Chancellor have all been vocal about the need to slash Stamp Duty with Boris Johnson himself referring to the current level of Stamp Duty rates as ‘absurd’.”Uncertainty removedJason Risholver, CEO of developer the Heronslea Group“We are delighted that The Conservatives have won, this is the best outcome for the UK property market. All the uncertainty surrounding the market and the lack of confidence in buyers will now quickly disappear as the two reasons why we are in a challenging market – Brexit and political uncertainty have gone. We now have a clear picture and direction for our country.” Andrews Property Stone Real Estate Chesterton General Election Jackson-stops Nick Leeming 2019 Benham and Reeves David Westgate flatfair December 13, 2019Nigel LewisWhat’s your opinion? Cancel replyYou must be logged in to post a comment.Please note: This is a site for professional discussion. Comments will carry your full name and company.This site uses Akismet to reduce spam. Learn how your comment data is processed.Related articles BREAKING: Evictions paperwork must now include ‘breathing space’ scheme details30th April 2021 City dwellers most satisfied with where they live30th April 2021 Hong Kong remains most expensive city to rent with London in 4th place30th April 2021 Home » News » Housing Market » Get ready for a post-election ‘turbocharged’ property market bounce previous nextHousing MarketGet ready for a post-election ‘turbocharged’ property market bounceEstate agents, developers and tech chiefs hail Conservative landslide as good news for housing, although many demand action on Stamp Duty.Nigel Lewis13th December 201905,374 Views
A new Oxford University research project has found that poorer nations are more active with regards to wildlife conservation than their better economically-developed counterparts.Researchers from Oxford’s Wildlife Conservation Research Unit (WildCRU) partnered with Panthera, the world’s only organisation dedicated to protecting wild cats, to assess the level of commitment of individual countries to protecting wildlife.The team created a Mega-Fauna Conservation Index (MCI) of 152 countries to assess their conservation footprint and created a benchmarking system which evaluated three key measures: a) the proportion of the country occupied by each mega-fauna species; b) the proportion of mega-fauna species range that is protected; and c) the amount of money spent on conservation relative to GDP.African countries topped the list, with Botswana, Namibia, Tanzania and Zimbabwe leading.The United States found itself at nineteenth, and a quarter of countries in Asia and Europe were classed as significantly underperforming.Panthera research associate Dr Peter Lindsey, who led the collaboration, told Cherwell: “This is the first attempt to try to compare the conservation efforts of different countries. We need to be able to compare efforts to create a floating benchmark so that the average effort is pulled up, especially as mega-fauna populations are dropping.”He added: “Mega-fauna act as a proxy for conservation efforts in general, hopefully in the future the study might be expanded to monitor marine conservation efforts.”Professor David Macdonald, Director of WildCRU and co-author of the paper said: “Every country should strive to do more to protect its wildlife. “Our index provides a measure of how well each country is doing, and sets a benchmark for nations that are performing below the average level, to understand the kind of contributions they need to make as a minimum.”The study also explains the reasons for this disparity in contributions to conservation. Mega-fauna are valuable assets and to many less affluent countries their existence provides both a national identity and an economic lifeline in the form of tourism, which provides a high proportion of the GDP of some African nations: for example in 2014, tourism contributed 17 per cent of Tanzania’s GDP.Dr Dawn Burnham, also of WildCRU, said “What really matters is the idea we have developed, rather than the detail: countries can be ranked in their commitment to conservation, and each country can and should strive to climb the rankings—the details of how the rank is calculated can surely be refined in future, but the idea of the ranking will endure”.Speaking about the future of the project, Dr Lindsey said: “We will be generally improving the study and making it as fair as possible. Our goal is to have an index that is published annually and the performance of countries regularly assessed.”At the 1992 Rio Earth Summit, developed nations promised to allocate at least $2 billion (USD) per year towards conservation in developing nations.However, current contributions from developed nations are just half of the proposed amount, $1.1 billion (USD) per year.
The Bakers’ Fair is to take place on Sunday, 19 October 2008, at Sheffield’s Don Valley Stadium. As the UK’s only autumn exhibition for progressive and professional independent bakery companies, cafés and coffee shops, it comes at a time when key purchasers will be making final decisions about the Christmas selling period, as well as looking ahead to 2009.The Richemont Club of Great Britain will be judging its fifth National Competition at the event, with competing classes putting forward a range of bakery products. It is a great opportunity for everybody in the industry to exhibit, including ingredient suppliers and manufacturers, flour millers, wholesalers, finished goods suppliers, cakes, pastry and biscuit suppliers and coffee, tea and soft drinks suppliers.The event is organised by British Baker’s publisher, William Reed Business Media. To reserve your stand, please contact Jennie Dick on 01293 846520 or email [email protected] Entry for trade visitors is free and it will be open between 9.30am and 4pm. See [http://www.bakersfair.co.uk] for more details.
Last weekend’s Brooklyn Comes Alive brought an almost overwhelmingly strong slate of exciting collaborations to Williamsburg, Brooklyn over the course of two full days of music across three venues, all within a few blocks of each other. Last Saturday evening, amid the musical madness of day 1 of the one-of-a-kind festival, fans were met with a musical “Sophie’s Choice”. A rare set from Rooster Conspiracy (a new improvisational Grateful Dead-inspired project featuring Eric Krasno, Reed Mathis, Todd Stoops, and Jay Lane), the official debut of Matador! Soul Sounds (a brand new band featuring Eddie Roberts, Alan Evans and vocalists Adryon De León & Kim Dawson) and a special jam session led by Trey Anastasio Band sax man James Casey were all scheduled to take place during the same early-evening time slot, forcing the festival crowd to make some tough decisions.Relive The Magic Of Brooklyn Comes Alive 2017 With This Set-By-Set Breakdown Of All The ActionWhile there were no wrong answers on this memorable weekend, and all three sets received rave reviews, the fans who chose James Casey & Friends were treated to a dark-horse candidate for one of the weekend’s best and most unique performances. Casey was scheduled to bring his band Animus Rexx to Brooklyn Comes Alive, but due to a visa issue, one of his band members got tied up overseas and couldn’t make it back to States for the scheduled performance. So instead, the week of the performance, James decided to gather together some friends for a jam session. Here’s what we learned: James Casey has some seriously badass friends.For the performance, Casey tapped Felix Pastorius, son of the legendary Jaco Pastorius, to fill bass duties, as well as Louis Cato and Randy Runyon to round out the core band. The set also featured guest appearances from Robert “Sput” Searight, Nate Werth, and more. The band touched on a variety of styles and hip covers, including material by Anderson .Paak and more. Casey’s Trey Anastasio Band cohort Natalie Cressman also made a memorable guest appearance to lend her vocals to another unexpected cover–hit EDM track “Wildfire” by SBTRKT.You can watch fan-shot footage of James Casey, Natalie Cressman, Felix Pastorius, and more performing SBTRKT’s “Wildfire” at Brooklyn Comes Alive below:
While war films date to the beginnings of cinema and the Spanish-American War, World War I’s magnification of the mutual impact of war and cinema on each other brought the relationship to an entirely new level. As the war that introduced modern technology into combat, World War I saw film and the moving image enlisted as instruments of surveillance and documentation. Away from the battlefront, propaganda films and newsreels worked to keep the civilian population informed and to incite them to join the fight.The Harvard Film Archive is proud to present a survey of films about World War I, which span several countries, decades and contexts, illustrating that the trauma of the war meant that as often as not, war films became anti-war films. If the conflict was not “the war to end all wars,” it nevertheless represented the end of the early modern age and the coming of an entirely new world, one in which cinema would have a central place.This program is presented in conjunction with a two-day conference at the Mahindra Humanities Center at Harvard, “In Our Time: The Great War at 100,” on February 12 and 13. For more information, visit their website.The series runs from Feb. 13 – March 2. Join the conversation with #GreatWarAt100
LAHORE, Pakistan (AP) — A defense lawyer says a Pakistani court has indicted the owner and editor-in-chief of the country’s largest independent group of newspapers and TV stations, along with two former government officials in a decades-old case related to allegations of tax evasion in a real estate purchase. Mir Shakilur Rehman’s Jang Group of Newspapers, which includes Geo TV, has been critical of the government. He was arrested in March 2020 by the National Accountability Bureau and released months later on bail. His lawyer says Rehman was indicted on Thursday in the city of Lahore. He’s accused of purchasing government land in violation of rules, a charge he denies.
More water Prepare the soil Many vegetable plants’ first days in the garden aren’t as seedlings poking through the soil, but as young plants that started life indoors. For those plants, a University of Georgia gardening expert says some “transplanting rules of thumb” can make your garden much more successful. Prepare the plants To make the transplanting proceed more smoothly, McLaurin said, prepare the soil first. “Try to do this just before you transplant, so the soil will be cool and moist,” he said. “If the soil is worked up and loose, it will make the root-to-soil contact much easier.” “Cradle that little root ball!” he said. “Keep as much soil as you can around it. That way, fewer root hairs will get exposed to air and die.” Damaging root hairs is inevitable in transplanting, he said. But try to keep this damage to a minimum. “Root hairs are what the plant takes up water and nutrients through,” he said. “They’re the feeders on the regular roots, and they’re so small you can’t see them.” Set the root ball carefully in the hole, fill in the soil and firm it well so the roots make good contact with the soil. Pour on the water Soak the plants in the flats thoroughly. That will help the soil and roots stay together as tightly as possible when you remove each plant from its container. Transplant on a cloudy, wind-free day if you can, McLaurin said. Or do it late in the afternoon when the sun has begun to set. Then there is less air-drying of the roots. Handle with care Then place a handful of compost or a teaspoon of 5-10-15 fertilizer into the planting hole. Always cover fertilizer with some soil. Putting the soil between the roots and fertilizer keeps the roots from being damaged by fertilizer burn. “Remember, this is in addition to that broadcasting of fertilizer you added earlier over the entire garden,” McLaurin said. Add fertilizer “Leaving the plants outside will harden them off and get them ready for transplanting,” said Wayne McLaurin, an Extension Service horticulturist with the University of Georgia Extension Service. “The longer the flats of plants have been outside, especially overnight,” he said, “the less shock the transplants will have to withstand.” Then give the transplants a good soaking. Direct the water flow around the base of the plant. But try not to get the water on the leaves and stems. “Water thoroughly,” McLaurin said. “Watering to the point of practically making mud ensures the best possible cementing of roots and soil and the least possible delay in new growth.” Young transplants need watering the first three or four days, he said, until they become established. This is especially true in March and early April when the wind can dry the plants and soil quickly.
Cover photo: ULTRA Festival The founder of the Sonus Festival in Zrće, Dalibor Oliver Zjačić, says that music festivals have become popular because they have been working on content that is recognized all over the world. “This holiday aspect is also important and in that sense Croatia has established itself as the largest festival destination in the world. Of the top 50 performers in the world, certainly 40 of them play on Sonus, while taking care of safety, which is very important for tourists. We work to keep our guests coming back from year to year”, Zjacic emphasized. “1. HRF Academy, is not the last. Today’s panel discussion has just begun the cycle of HRF Academy, which aims to inform, educate and speak about strategically important topics in Croatia, current events, challenges and important media messages that promote media culture.”, Announced the president of the Croatian Radio Forum Silvija Londero Šimleša. Joe Bašić, who brought Ultra Europe to Croatia, believes that young people are looking for an experience that they can only experience at music festivals. “I often call Ultra the Olympic Games in music and I think we should be very proud and happy to have such an event in Croatia. We have become a lure for people from all over the world, and this year we will have about 150 thousand visitors in seven days”, Said Basic. The President of the Board of the Exit Foundation, Ivan Petrović, who is behind the Sea Star Festival in Umag, points out that the industry of music festivals around the world is growing, because such events are part of growing up. “What used to be summer vacations are now music festivals. Sea Star is one of the main festivals in Croatia, but the only one held in the pre-season, which we are especially proud of because it is much harder to make a festival then, than in the peak season”, Said Petrovic. Director of the Strategic Communications Department of the CNTB, Nenad Gorjanović, points out that our country benefits a lot from music festivals that complement the Croatian tourist offer. “We are especially pleased when festivals take place throughout the year because we all have the same goal – to create a tourist year, not just the main season. Music festivals can often be the main motive for tourists, so their impact on tourist traffic is very strong. Festivals, in addition to creating quality content, significantly contribute to the image of Croatia as an attractive and dynamic destination”, Said Gorjanovic. Kaptol Boutique Cinema hosted a multimedia 1st HRF Academy on “The impact of music festivals on tourism”, organized by the Croatian Radio Forum, in partnership with the Ministry of Tourism and the Croatian Tourist Board, and a panel discussion brought together experts from tourism and music festivals. The director of the Zagreb Tourist Board, Martina Bienenfeld, emphasizes the importance of music festivals. “This is best seen in the example of Zagreb, which has various festivals throughout the year. Guests who come to INmusic in June stay longer in Zagreb, and often spend their holidays in other parts of Croatia. At the same time, other consumption is realized, and not only festival consumption, from which the City of Zagreb benefits greatlySaid Bienenfeld.